{"id":505,"date":"2015-11-09T11:00:16","date_gmt":"2015-11-09T10:00:16","guid":{"rendered":"http:\/\/www.dhufire.org\/?page_id=505"},"modified":"2015-11-09T11:02:03","modified_gmt":"2015-11-09T10:02:03","slug":"nidaa-mikail","status":"publish","type":"page","link":"https:\/\/www.dhufire.org\/?page_id=505","title":{"rendered":"Nidaa Mikail"},"content":{"rendered":"<p><\/p>\n<p style=\"text-align: justify;\"><strong>Molecular imaging of myocardial ischemic memory in rat, using <sup>99m<\/sup>Tc-fucoidan<\/strong><\/p>\n<p style=\"text-align: justify;\">Mikail N<sup>1,2,3<\/sup>, Anizan N<sup>3<\/sup>, Louedec L<sup>2,3<\/sup>, Aerts J<sup>1,2,3<\/sup>, Al Shoukr F<sup>1,3<\/sup>, Michel JB<sup>2<\/sup>, Letourneur D<sup>2<\/sup>, Chauvierre C<sup>2<\/sup>, Le Guludec D<sup>1,2,3<\/sup>, Rouzet F<sup>1,2,3<\/sup><\/p>\n<p style=\"text-align: justify;\"><sup>1 <\/sup>Nuclear Medicine Department, Bichat-Claude Bernard Hospital;\u00a0<sup>2 <\/sup>LVTS (INSERM U1148) Team 4, Cardiovascular Bioengineering;\u00a0<sup>3<\/sup> F\u00e9d\u00e9ration de Recherche en Imagerie Multimodale (FRIM)<\/p>\n<p style=\"text-align: justify;\">Partners :<\/p>\n<ul style=\"text-align: justify;\">\n<li>LVTS (Inserm U1148)<\/li>\n<li>F\u00e9d\u00e9ration de Recherche en Imagerie Multimodale (FRIM, Univ. Paris Diderot)<\/li>\n<li>Laboratoire de Bio-Ing\u00e9nierie des Polym\u00e8res, Universit\u00e9 Paris 13.<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">Mail. : <a href=\"mailto:nidaa.mikail@gmail.com\">nidaa.mikail@gmail.com<\/a><\/p>\n<p style=\"text-align: justify;\"><strong>Purpose: <\/strong><\/p>\n<p style=\"text-align: justify;\">The retrospective identification of a remote acute coronary syndrome is a diagnostic challenge. Because adhesion molecules such as selectins persist on the endothelial surface after ischemia has resolved, they represent a tissue \u201cimprint\u201d of the prior ischemic event, referred to as ischemic memory. <sup>99m<\/sup>Technetium labelled Fucoidan (<sup>99m<\/sup>Tc-fucoidan) is an imaging agent of selectins. In this study we aimed to evaluate the ability of <sup>99m<\/sup>Tc-fucoidan SPECT to detect myocardial ischemic memory in various settings regarding occlusion duration and reperfusion delay.<\/p>\n<p style=\"text-align: justify;\"><strong>Methods: <\/strong><\/p>\n<p style=\"text-align: justify;\">Transient myocardial ischemic events have been induced in male Wistar rats (duration from 20 min to 5 min). <sup>99m<\/sup>Tc-fucoidan SPECT was performed after reperfusion delays of 2h, 6h and 24h. Animals were then sacrificed and their heart explanted, followed by autoradiography, histology (hematoxylin-eosin staining) and immunostaining (P-selectin).<\/p>\n<p style=\"text-align: justify;\"><strong>Results: <\/strong><\/p>\n<p style=\"text-align: justify;\">Visual detection of 99mTc-fucoidan myocardial uptake is presented in Table. Quantitative assemment of myocardial tracer uptake on SPECT acquisitions was (according to ischemia duration): 20 min= 2.3\u00b10.4 [1.9-2.7]; 10min= 3.8\u00b10.2 [3.5-4.0]; 5min= 2.2\u00b10.4 [1.9-2.9]). Areas of <sup>99m<\/sup>Tc-fucoidan uptake matched with endothelial expression of P-selectin.<\/p>\n<p style=\"text-align: justify;\"><strong>Conclusion: <\/strong><\/p>\n<p style=\"text-align: justify;\">This study shows that brief coronary occlusions (5 minutes) followed by reperfusion durations up to 24 hours are associated with P-selectin endothelial expression, and detectable <em>in vivo<\/em> by <sup>99m<\/sup>Tc-fucoidan SPECT. Therefore, <sup>99m<\/sup>Tc-fucoidan is a promising agent for detection of ischemic memory. It will be soon available as a GMP radiolabelling kit for clinical translation.<\/p>\n<p style=\"text-align: justify;\"><strong>Table<\/strong><br \/>\n<a href=\"http:\/\/www.dhufire.org\/wp-content\/uploads\/2015\/11\/Table-N.-Mikail.png\"><img loading=\"lazy\" class=\"alignleft wp-image-507 size-full\" src=\"http:\/\/www.dhufire.org\/wp-content\/uploads\/2015\/11\/Table-N.-Mikail.png\" alt=\"Table N. Mikail\" width=\"813\" height=\"202\" srcset=\"https:\/\/www.dhufire.org\/wp-content\/uploads\/2015\/11\/Table-N.-Mikail.png 813w, https:\/\/www.dhufire.org\/wp-content\/uploads\/2015\/11\/Table-N.-Mikail-300x75.png 300w\" sizes=\"(max-width: 813px) 100vw, 813px\" \/><\/a><\/p>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Molecular imaging of myocardial ischemic memory in rat, using 99mTc-fucoidan Mikail N1,2,3, Anizan N3, Louedec L2,3, Aerts J1,2,3, Al Shoukr F1,3, Michel JB2, Letourneur D2, Chauvierre C2, Le Guludec D1,2,3, Rouzet F1,2,3 1 Nuclear Medicine Department, Bichat-Claude Bernard Hospital;\u00a02 LVTS <a class=\"more-link\" href=\"https:\/\/www.dhufire.org\/?page_id=505\">Lire la suite <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":473,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_analytify_skip_tracking":false,"_links_to":"","_links_to_target":""},"_links":{"self":[{"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/pages\/505"}],"collection":[{"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.dhufire.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=505"}],"version-history":[{"count":2,"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/pages\/505\/revisions"}],"predecessor-version":[{"id":508,"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/pages\/505\/revisions\/508"}],"up":[{"embeddable":true,"href":"https:\/\/www.dhufire.org\/index.php?rest_route=\/wp\/v2\/pages\/473"}],"wp:attachment":[{"href":"https:\/\/www.dhufire.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}